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| Purification and Toxicological Studies of HaSNPV Chitinase |
| XI You-wei, XIA Tian, ZHAO Ya-ling, JIA Jing-fang |
| School of Chemical Engineering, Northwest University, Xi’an 710069, China |
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Abstract Recombinant bacterium GS115-pPIC 9k-ChiA 4.0 fermented chitinase, which was purified by ammonium sulfate precipitation, dialysis , DEAE Sepharose Fast Flow ion exchange chromatography and Sephadex G-100 gel filtration chromatography, obtained electrophoretically pure chitinase. Rate of recovery was 19.64%, and purification factor was 17.74. Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HaSNPV) chitinase toxicological studies carried out by contact toxicity and stomach toxicity, chitinase liquefied Plutella xylostella larvae cell and caused death. The effect and the concentration of chitinase is proportional to time, and the effect caused by stomach toxicity was more intense than by action of contact toxicity. The result of antibacterial experiments which were performed on Helminthosporium sativum, Tomato botrytis cinerea, Tobacco brown spot, and Colletotrichum gloeosporioides, indicated that chitinase can inhibit fungal by enzymatic hydrolysis of chitin in fungal cell wall.
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Received: 21 September 2010
Published: 18 February 2011
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