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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (5): 121-125    DOI:
    
Progress of the Study on the Method of DNA Extraction from Genetically Modified Food
CAI Cui-xia, XIAO Wei-wei, KANG Wen-jie, MA Wen-li
Institute of Genetic Engineering Southern Medical University, Guangzhou 510515, China
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Abstract  

In order to meet consumers' right to know for genetically modified(GM) food,it is very important to establish accurate, rapid and efficient GM food detection technology. The quality and quantity of the DNA is considered to be a prerequisite factor for the GM food detection.Several DNA extraction methods used at home and abroad in the recent years are reviewed, such as hexadecyl trimethyl ammonium bromide (CTAB) methods, dodecyl sulfate, sodium salt(SDS) methods,polyvinylpyrrolidone( PVP )methods,medium purify methods,chelating resin methods etc. The advantages, disadvantages, application range of the above methods are comprehensively summarized, along with analyzes the problems should be paid attention to and the GM food DNA extraction developing direction, which will offer operative information for rudimenting workers in DNA extraction.

Key words： Genetically modified food      DNA extraction     
Received: 21 December 2010      Published: 27 May 2011
ZTFLH:  R34  
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CAI Cui-xia, XIAO Wei-wei, KANG Wen-jie, MA Wen-li. Progress of the Study on the Method of DNA Extraction from Genetically Modified Food. China Biotechnology, 2011, 31(5): 121-125.

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https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I5/121


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