|
|
Screening of HIV-1 p24 Binding Peptides from Phage Display Peptide Library |
WANG Zi-ye, FENG Juan, MA Xue-mei, WANG Ming-lian, ZHONG Ru-gang |
College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China |
|
|
Abstract A phage display peptide library was screened by targeting recombinant p24 antigen. After three rounds of panning, eleven phage clones were chosen to test the binding affinity by ELISA, and ten of them were positive. DNA sequencing showed that nine of the positive clones display a unique sequence FTTRANA which has high affinity with HIV-1 p24 antigen. To improve the condition of phage ELISA, phage number and blocking buffer have been optimized, phage number ranged from 8.13×1011 to 1.02×1014 pfu/well and 0.5%BSA was highly recommended. Therefore, in this study, peptides combined with p24 antigen were obtained which might be useful in p24 antigen assay.
|
Received: 20 December 2010
Published: 27 May 2011
|
|
|
|
[1] 熊祝嘉. ELISA检测p24抗原用于诊断HIV感染的研究现状. 实用医技杂志, 2007, 14(2): 166-168. Xiong Z J. Journal of Practical Medical Techniques, 2007, 14(2): 166-168.
[2] 郑磊, 贾立永, 干宁, 等. 人免疫缺陷病毒感染的实验室检测及进展. 分子诊断与治疗杂志, 2010, (4): 284-288. Zheng L,Jia L Y,Gan N, et al. Journal of Molecular Diagnosis and Therapy, 2010, (4): 284-288.
[3] Filice G, Soldini L, Orsolini P, et al. Detection of antibodies to p24 and gp41 epitopes of HIV by ELISA using the recombinant core protein (p24) and envelope synthetic protein (gp41). Microbiologica, 1991, 14(1): 1-7.
[4] Woo M K, Heo C K, Hwang H M, et al. Optimization of phage-immobilized ELISA for autoantibody profiling in human sera. Biotechnol Lett, 2010, 33(4):655-661.
[5] Smith G P. Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science, 1985, 228(4705): 1315-1317.
[6] Matsumoto M, Horiuchi Y, Yamamoto A, et al. Lipopolysaccaride-binding peptides obtained by phage display method. J Microbiol Methods, 2010, 82(1): 54-58.
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|