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| Fumaric Acid Fermentation by Immobilized Rhizopus oryzae with Biofilm Reactor |
| LIU Qiang, XU Qing, LI Shuang |
| College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China |
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Abstract The production of fumaric acid was optimized using immobilized Rhizopus oryzae with biofilm reactor and neutralized with Na2CO3 in 5L stirred tank reactor. Visualization method (IVOS) was used to optimize the process for fumaric acid production. Under the optimized condition (80g/L total glucose),the best results were fumaric acid titer of 21.10g/L, the volumetric productivity of 0.25 g/L·h and the yield of 28%, respectively; in the continuous batch fermentation condition(40g/L total glucose), the best results were fumaric acid titer of 10.8 g/L,yield and volumetric productivity of fumarate were 27% and 0.36 g/L·h,respectively. Owing to the limitation of specific surface area of biofilm and its blocking mass transfer, biofilm reactor could not give an effective fumaric acid production. Future prospects for improvement of fumaric acid production include developing new methods for immobilized cell and bioreactor.
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Received: 06 November 2013
Published: 25 February 2014
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