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| Cloning and functional characterization of cDNA sequence encoding the MAP kinase kinase AtPBS2 gene of the fungus Alternaria tenuissima |
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Abstract An Alternaria tenuissima cDNA yeast expression library was constructed The MAPK kinase PBS2 was isolated from an Alternaria tenuissima cDNA expression library, designated as AtPBS2. It has a size of 2,492 bases in length, encoded a protein of 683 amino acids. The AtPbs2p amino acid sequence shows 52%、52%、49% and 47% identities with AfPbs2p (XP_752961) of Aspergillus fumigatu、MGCH7 (XP_001522946) of Magnaporthe grisea and ScHog1p (EDN63254) of Saccharomyces cerevisiae, respectively. AtPBS2 cDNA sequences could complement the functions of S. cerevisiae ScPBS2 genes in sodium tolerance, suggesting a functional HOG pathway exists in A. tenuissima, AtPBS2 gene was involved in the stress adaptation regulation of A. tenuissima.
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Received: 04 January 2009
Published: 02 July 2009
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