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| Breeding of Yeast Fusant for Efficient Ethanol Fermentation from Xylose |
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Abstract Yeast strains with improved ethanol yield are important for efficient bioconversion of lignocellulosic biomass for fuel ethanol. In this study, Candida shehatae CICC1766 was adapted to 4% (v/v) ethanol, and then subjected to UV mutagenesis. One respiration deficient mutant Rd-5 with improved xylose fermentation capability was selected. Protoplasts of Rd-5 were inactivated by UV treatment, followed by the PEG-mediated protoplast fusion with a Saccharomyces cerevisiae strain with good ethanol-fermenting capability. The xylose fermenting capability of the fusants was investigated, and the fusant F6 demonstrated good ethanol fermentation performance, producing 18.75 g•L-1 ethanol from 50 g•L-1 xylose with an ethanol yield of 0.375 or 73.4% of its theoretical value of 0.511. Comparing with its parent Candida shehatae strain, the ethanol yield of F6 was increased by 28%.
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Received: 26 May 2008
Published: 02 July 2009
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