Abstract Straw contains large quantities of lignocelluloses that the structural is stability and hardly degraded. The influence of the different mixtures of five strains that can degrade lignocelluloses were studied using mixture design. Regression model on microorganism composition and degrading rate of lignocelluloses and content of reducing sugar was established. The predictable degrading rate of cellulose reached the maximum, 35.75%, the maxim predictable degrading rate of lignin was 27%, the most predictable production of reducing sugar was 3.39mg/g.Based on these response values satisfied all expectation were optimized, and the most excellent combinations of Bacillus Subtilis, Bacillus Licheniformis, Canadian krusei, Trametes versicolor, Phaerochaete chrysosporium were 12.1%,10%, 27.2%, 10.6%, and 40%, respectively.
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