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| Tandem repeat Expression and inhibitory activity of a β-lactamase inhibitory peptide |
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Abstract β-lactamase inhibitor research is popular for its potential onβ-Lactam antibiotics resistant strain. A β-lactamase binding peptide SIPIS04-01 was obtained by the yeast two-hybid system. In vitro assay showed that it can inhibit the β-lactamase activity. In order to improve the expression level of the recombinant peptide, a two-copy expression plasmid pYG563 was constructed by random orientation tandem repeat method After codon modification, this two-copy plasmid was successfully expressed and the product was increased by 48.4％ than that of one-copy plasmid. Purified peptide showed inhibitory activity against TEM-1 β-lactamase in vitro and the inhhibitory constant Ki was measured.
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Received: 05 February 2009
Published: 10 May 2010
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| Fund: the National Natural Science Foundation of China |
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