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| The Artificial Aptazyme Based Riboswitch |
| WANG Jia-wen1,2, FENG Jing-xian1,2, LIN Jun-sheng1,2, DIAO Yong1,2 |
1. Institute of Molecular Medicine, Quanzhou 362021 China;
2. School of Biomedical Sciences Huaqiao University, Quanzhou 362021, China |
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Abstract The artificial aptazyme based riboswitch has emerged as a powerful tool for regulating gene expression in recent years. The most common aptazyme consists of a hammerhead ribozyme and an aptamer, it is simply a piece of structured RNA and easy to design. As a cis-acting element, aptazyme riboswitch can recognize specific targets and exert effective influence on translation without the help of protein co-factor through irreversible self-cleavage in vivo, which makes it a versatile platform functions in various cells. The most popular approach to generate an aptazme based riboswitch is to rationally cojoin a ribozyme to an aptamer and screen with a high throughout method after integrated into mRNA. With considerable magnitude of on-off control and rapid induction as well as concise regulation, this riboswitch will have important application in in vivo sensing, gene therapy and biological processor.
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Received: 03 December 2013
Published: 25 February 2014
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