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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (03): 35-38    DOI:
    
Cytotoxic Study of Dendritic Cell Modified by PEG10 Gene on Liver Cancer Cells
LI Ling-ling1, ZHOU Wei2
1. Biochemistry Engineering Department, Chongqing Industry &Trade Polytechnic, Chongqing 408000,China;
2. Institute of Immunology,Third Military Medical University, Chongqing 400038,China
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Abstract  

Objective:To explore the effect of dendritic cell modified by PEG10 gene for liver cancer. Methods:Dendritic cells originated from the HLA-A2+ PBMCs were transfected with recombinant adenovirus containing PEG10 full length cDNA, and the modified DC were utilized to generate specific cytotoxicity T lymphocytes (CTL). The activity was analyzed by standard 51Cr releasing assay and ELISPOT. Results:The transfected DCs could elicit specific CTLs, which could secrete IFN-γ and lyse liver tumor cells. Conclusions:The PEG10 recombinant adenovirus transfected DCs may provide a new therapeutic strategy for hepatocellular carcinoma.



Key wordsPEG10      Dendritic cell      Hepatocellular carcinoma      Imprinted gene     
Received: 20 October 2010      Published: 01 April 2011
ZTFLH:  R735  
Cite this article:

LI Ling-ling, ZHOU Wei. Cytotoxic Study of Dendritic Cell Modified by PEG10 Gene on Liver Cancer Cells. China Biotechnology, 2011, 31(03): 35-38.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I03/35

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