|
|
|
| Expression and Activity Analysis of Two Anti-microbial Peptides from Aquatic Animals |
| SHU Mei, XU Yang, XU Xi, TU Zhui |
| State Key Laboratory of Food Science and Technology, Sino-Germany Joint Research Institute, Nanchang University, Nanchang 330047, China |
|
|
|
Abstract Anti-microbial peptides sequence from two kinds of aquatic animals have been cloned into the prokaryotic expression vector pGEX-4T-1, constructing two fusion protein expression vector pGEX-Y18 and pGEX-CEC1,which were transformed into Escherichia coli Rosetta to express,and the existent form of expression fusion protein mainly in inclusion body. Fusion protein was renatured,and cleaved to produce antimicrobial peptides Y18 and CEC1. Antimicrobial tests showed that not only fusion protein GST-Y18 and GST-CEC1 but also antimicrobial peptide Y18 and CEC1 can effectively restrain the growth of E. coli DH5α、S. aureus、B. subtilis. and S. cerevisiae.
|
|
Received: 16 August 2010
Published: 18 February 2011
|
|
|
|
|
| [1] Steiner H, Hultmark D,Enqstrom A, et al. Sequence and specificity of two antibacterial proteins involved in insect immunity. Nature, 1981, 292 (5820): 246-248.
[2] Rathinakumar R, Walkenhorst W F, Wimley W C, et al. Broad-spectrum antimicrobial peptides by rational combinatorial design and high-throughput screening: the importance of interfacial activity. J Am Chem Soc, 2009, 131 (22):7609-7617.
[3] Huang W S,Li S Q, Cai L,et al.Cloning, sequence analysis and gene organization of antimicrobial peptide hepcidin gene from Nile Tilapia(Oreochromis niloticus). JXMU Natural Science,2007,46(3):390-395.
[4] Basanez G, Shinnar AE,Zimmerberg J. Interaction of hagfish cathelicidin antimicrobial peptides with model lipid membranes.FEBS Lett,2002,532(1-2):115-120.
[5] Pukala T L, Bowie J H, Masell V M, et al. Host-defence peptides from the glandular secretions of amphibians:structure. Nat Prod Rep,2006, 23 (3): 368-393.
[6] Douglas Susan E, Patrzykat Aleksander. Identification,structure and differential expression of novel pleurocidins clustered on the genome of the winter flounder, pseudopleuronectes americanus. Eur J Biochem,2003, 270(18)3720-3730.
[7] Reddy K V, Yedery R D,Aranha C, et al. Antimicrobial peptides:premises and promises. Int J Antimicrob Agents,2004,24 (6) :536-547.
[8] Lai Y, Gallo R L. AMPed up immunity:how antimicrobial peptides have multiple roles in immune defense. Trends Immunol, 2009, 30 (3): 131-141.
[9] Oren Z, Shai Y. A class of highly potent antibacterial peptides derived from pardaxin, a pore-forming peptide isolated from Moses sole fish Pardachirus marmoratus. Biochemisry ,1996,237(1): 303-310.
[10] Pouny Y, Rapaport D. Interaction of Antimicrobial dermaseptin and its fluorescently iabeled analogues with phospholipid membranes.Biochemistry,1992,31(49):12416-12423.
[11] Paul Y, Kassebaum C, Lazarovici P, et a1.Translocation of acylated pardaxin into cells .FEBS Lett, 1998, 440 (122):131-134.
[12] Liu B,Lu Y, Zhang Z, et a1. Identification and antifungal assay of a wheat β-1, 3-glucanase. Biotech Lett,2009,31 (7) : 1005-1010.
[13] 陆海荣,李国栋,吴宏宇,等.抗菌肽GK1在大肠杆菌中的融合表达.生物工程学报,2008,24(1):21-26. Lu H R,Li G D,Wu H Y,et al.Chin J Biotech,2008,24(1):21-26.
[14] 路妍,刘宝业,井金学,等。抗菌肽Rs-AFP2的原核表达及抑菌活性.植物遗传资源学报,2010,11(1):84-88. Lu Y, Liu B Y,Jin J X, et al. JPGR,2010,11 (1): 84-88.
[15] 张贞,柯涛,周玉玲,等.利用融合蛋白 EDDIE 在大肠杆菌中高效表达抗菌肽Cecropin AD.生物工程学报,2009,25(8):1247-1253. Zhang Z, Ke T, Zhou Y L,et al.Chin J Biotech,2009, 25(8): 1247-1253. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
