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Abstract bjective ：To look for the correlated gene of CNE2 cell after bax gene transfection. Method: In CNE2 cell after bax gene transfection analysed by mRNA defference to demonstrate, and DNA sequencing was performed, and small amount of PCR products were used as probes for Northern blot after labeled with radioisotope. Result: By mRNA differential display technique, ten differential expression bands were found in CNE2 cell, four bands of which were induced by pSFFV-bax-neo and the others inhibited. The four cDNA framents were to reamplify by PCR selected from the inhibited bands and expressions in CNE2 cell were found by Northern blot. Conclusion：Bax gene can induce the expression of some genes in CNE2 cell, whereas it can also inhibit the expression of some genes. By selecting two lengthened primers and adding T7RNA polymerase promoter to the nondT anchoring primers, it can overcome the disadvantags of excessive PCR products and pseudopositive bands in mRNA differential display technique, Thus PCR products can be sequenced directly.
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Received: 09 February 2006
Published: 25 February 2006
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