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| Function of p21-activated kinase 2 in oocyte maturation |
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Abstract Study the function of p21-activated kinase 2 (PAK2) in Xenopus oocyte maturation. Inject PAK2-N teminal (PAK2-NT) mRNA to Xenopus oocyte,and observe germinal vesicle breakdown by fluorescence microscope. To further observe the changes of F-actin and spindle, confocal microscopy with time-lapse was employed.Compared with the controls, injection of PAK2-NT mRNA caused no visible changes in oocyte morphology nor affected progesterone-induced germinal vesicle breakdown,but block cytokinesis and polar body formation. Maybe PAK2 involves in Xenopus oocytes maturation.
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Received: 14 December 2006
Published: 25 June 2007
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