The Fatty Acid Methyl Ester Profiling of Vibrio cholerae in China

China Biotechnology

2007, Vol. 27

Issue (6): 15-21 DOI:

The Fatty Acid Methyl Ester Profiling of Vibrio cholerae in China

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Abstract

Objective Evaluation the application value of Fatty acid methyl ester (FAME) method in strain identification and relationship judgment. Methods 194 V. cholerae strains belonging to the two main pathogenic seragroups, El Tor isolated since 1961 and O139 since 1992 in China, were selected and fatty acid methyl ester were extrcted. Microbial identification system of MIDI company was used to analysis the data. Results Thirteen fatty acids were common components contained in all of the tested V. cholerae strains. The coincidence of special judgment was 88.6%. There was no obvious separable group in 2-D plot analysis. Conclusion FAME method is useful in rapid identification of Vibrionaceae strains and in assistant field isolation of V. cholerae. It can be used to judge the relationships of strains in small outbreaks, but it cannot differential the subgroups of V. cholerae.

Key words： Vibrio cholerae fatty acid typing

Received: 30 January 2007 Published: 25 June 2007

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Cite this article:

. The Fatty Acid Methyl Ester Profiling of Vibrio cholerae in China. China Biotechnology, 2007, 27(6): 15-21.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I6/15

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