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| Expression of XYL1 of Candida tropicalis in Pichia pastoris and Xylitol Fermentation by Immobilizing Cells |
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Abstract XYL1 gene, which encodes xylose reductase with dual coenzyme activity from Candida tropicalis, was transformed into Pichia pastoris X-33 by expression vector pGAPZB. The recombination strain was immobilized in Ca-alginate beads and fermentation characterization is studied using corn cob hydrolysates. Fermentation conditions were as follow: initial pH value 6.0, 30℃, initial cell concentration of 20%, the Liquid volume of 28%, rotation speed 130r/min.The average xylitol yield was 37.5% on the optimum condition. This result is expected to provide a new alternative method for producing xylitol on a large scale by bioconversion.
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Received: 15 December 2008
Published: 02 July 2009
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Cite this article:
LI Jing, HE Xuan, ZHANG Fei-Wei, SU Yan-Qiu, HONG Gong, LU Fang-Hui, CHEN Tie-Lin, CAO Yi, JIAO Dai-Rong. Expression of XYL1 of Candida tropicalis in Pichia pastoris and Xylitol Fermentation by Immobilizing Cells. China Biotechnology, 2009, 29(06): 58-62.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2009/V29/I06/58
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| ［1］ 鲍晓明, 郑华军, 秦玉静,等. 酿酒酵母Saccharomyces cerevisiae重组菌株木糖醇发酵的初步研究. 工业微生物，2000, 30(2): 13~18
Bao X M, Zheng H J, Qin Y J. Industrial Microbiology, 2000, 30(2): 13~18
［2］ Jung-Kul Lee, Bong-Seong Koo, Sang-Yong Kim.Cloning and characterization of the xyl1 gene,encoding an NADH-preferring xylose reductase from Candida parapsilosis, and its functional expression in Candida tropicalis. Applied and Environmental Microbiology,2003, 69(10): 6179~6188
［3］ Maitreyee Banerjee. Kinetics of ethanolic fermentation of D-xylose by Klebsiella pneumoniae and its mutants. Appl Environ Microbiol, 1989, 55(5): 1169~1177
［4］ Lynda S Lacis, Hugh G Lawford. Effect of growth rate on ethanol production by Thermoanaerobacter ethanolicus in glucose or xylose-limited continuous culture. Biotechnol Lett, 1988, 10(8): 603~608
［5］ Wene E G, Anotonopoulos A A. D-xylose fermentation by Fusarium oxysporum strains. Biomass,1988, 17(1): 13~19
［6］ Yokoyama S I, Kinoshita Y, Suzuki T, et al. Cloning and sequencing of two D-xylose reductase genes (xyrA and xyrB) from Candida tropicalis. Journal of Fermentation and Bioengineering,1995, 80(6): 603~605
［7］ Yokoyama S I, Suzuki T, Kawai K, et al. Purification,characterization and structure analysis of NADPH dependent D-xylose reductases from Candida tropicalis. Journal of Fermentation and Bioengineering,1995, 79(3): 217~223
［8］ 廖翀, 白林含, 阮琨,等. 热带假丝酵母XYL1基因的克隆及序列分析. 四川大学学报,2006, 43(1): 228~231
Liao C, Bai L H, Ruan K, et al. Journal of Sichuan University (Natural Science Edition),2006, 43(1): 228~231
［9］ 侯爱华, 鲍晓明, 杨国梁. 外源基因在酵母中稳定表达的策略及研究进展. 微生物学杂志,2002, 22(4): 42~44
Hou A H, Bao X M, Yang G L Journal of Microbiology,2002, 22(4): 42~44
［10］ Primrosse S B, Derbyshire P, Jones I M, et al. Hereditary instability of recombinant DNA molecules. Soc Gen Microbial,1983,10: 63~67
［11］ Serienc F, Campbell J L, Bailey J E. Analysis of unstable recombinant Saccharomyces cerevisiae population growth in selective medium. Biotechnol Bioeng, 2004, 28(7): 996~1006
［12］ 欧阳立明, 张惠展, 张嗣同. 巴斯德毕赤酵母的基因表达系统研究进展. 生物化学与生物物理进展,2000, 27(2): 151~154
Ouyang L M, Zhang H Z, Zhang S L. Progress in Biochemistry and Biophysics,2000,27(2): 151~154
［13］ F. 奥斯伯，R.布伦特，R.E.金斯曼等. 精编分子生物学实验指南.北京:科学出版社,1998.22~23；332
F. Ausubel, R. Brent, R. E. Kingston et al. Short Protocols in Molecular Biology.Beijing:Science Press,1998.22~23,332
［14］ Ho N W Y, Chen Z T, Brainard A P,et al. Successful design and development of genetically engineered Saccharomyces yeasts for effective cofermentation of glucose and xylose from cellulosic biomass to fuel ethanol. Advances in Biochemical Engineering Biotechnology,1999, 65: 163~192
［15］ 何嘉波,吴宇澄. 固定化细胞转化木糖为木糖醇的研究. 江苏食品与发酵,2000, (4): 4~7
He J B, Wu Y C. Jiangsu Food and Fermentation,2000(4):4~7
［16］ 王晓霞,方伯山,李雯君. 木糖还原酶基因在大肠杆菌中活性表达.华侨大学报,2008, 29(3): 383~386
Wang X X, Fang B S, Li W J. Journal of Huaqiao University(Natural Science),2008, 29(3): 383~386
［17］ 万宁, 李伟成, 方伯山. 细胞粗提液中木糖还原酶的催化特性考察. 华侨大学学报,2001, 22(2):194~198
Wan N, Li W C, Fang B S. Journal of Huaqiao University(Natural Science),2001, 22(2): 194~198
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