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| Clinical Application of Rapid Detection of Pathogen of Gas Gangrene |
| TANG Ling-ling, SHI Yu-ling, WANG Lu-xia, LI Lin-hai, SUN Zhao-hui, CHEN Li-dan, XU Shao-shan |
| General Hospital of Guangzhou Command, Guangzhou 510010, China |
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Abstract Objective: To establish a rapid, sensitive and specific real-time PCR method for detection of Clostridium perfringens (C.perf) and application in clinic. Method: The C.perf-specific primers and probe were designed through a specific primer designing program based on C.perf-specific sequence, DNA of secretion and liquor puris were tested with this assay. To confirm sensitivity, speedability and specificity of this method compared with bacterial culture. Results: The best forward and reverse primers concentration were 0.45μmol/L and 0.15μmol/L respectively, the best probe concentration was 0.3μmol/L.This technique had good sensitivity,had no consensual reaction with other 21 bacterium species.The result of PCR accorded with that of the culture. Conclution:. The Taqman PCR assay, especially in the war, can be used for the rapid, sensitive and specific detection and enumeration of Clostridium perfringens from secretion and liquor puris with a short enrichment.
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Received: 13 September 2010
Published: 25 December 2010
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