Characteristics of HEK293 Cells Growth and Metabolism under Carrier-Free Immobilization Culture Mode

China Biotechnology

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Characteristics of HEK293 Cells Growth and Metabolism under Carrier-Free Immobilization Culture Mode

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Abstract By using the cell density, cell viability, size distribution of cell aggregates，specific consumption rate of glucose (qglc), specific production rate of lactate (qlac), lactate transform rate (Ylac/glc) and amino acids utilization as the evaluation indexes, the growth and metabolism of HEK293 cells under carrier-free immobilization culture mode were examined and compared with those of HEK293 cells cultured in static tissue flasks. It was found that HEK293 cells grown as suspended cell aggregates in spinner flasks maintained the basic growth and metabolism characteristics of HEK293 cells in stationary anchored culture, and HEK293 cells under carrier-free immobilization culture mode as suspended aggregates in stirred bioreactor facilitate perfusion performance and increase unit productivity. Cultivation of HEK293 cells in carrier-free immobilization culture mode is a potential and to be further improved mammalian cells culture technique.

Key words： metabolism

Received: 09 September 2005 Published: 25 February 2006

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. Characteristics of HEK293 Cells Growth and Metabolism under Carrier-Free Immobilization Culture Mode. China Biotechnology, 2006, 26(02): 20-24.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I02/20

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