The Research on Expression and Purification of a Chimeric anti-p185 antibody

China Biotechnology

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The Research on Expression and Purification of a Chimeric anti-p185 antibody

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Abstract TThe Her-2 proto-oncogene encodes a 185kD transmenbrane glycoprotein p185 which has intrinsic tyrosine kinase activity. It is overexpressed in several malignant human tumors like breast cancer. We have constructed a chimeric antibody by assembling a single-chain Fv antibody and a human IgG1 Fc fragment. This chimeric antibody reacts with tumor surface antigen p185c-erbB-2 specifically. In order to put the antibody into clinical application, we use two steps purification method to attain the antibody's purity more than 95%. Then we found both the lyophilized pharmaceutical formulations of the antibody. The formulations can keep the stability and activity of the antibody for at least 1 year. These results were the foundation of the chimeric antibody for cancer therapy.

Key words： Cation exchange purification Stability Chimeric antibody Affinity purification

Received: 27 April 2006 Published: 25 September 2006

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. The Research on Expression and Purification of a Chimeric anti-p185 antibody. China Biotechnology, 2006, 26(09): 32-37.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I09/32

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