中国生物工程杂志

The completed DNA of 1.7 kb HA gene was prepared from the cDNA by RT-PCR. The fragment was subcloned into the pFastBacHTa donor plasmids. the recombinant pFastBacHTa was cut by restriction enzyme and sequenced. then The purified plasmid DNA was transform into DH10Bac E. coli for transposition into the bacmid and the colonies was identified by blue/white selection. The recombinant bacmid was verified by PCR analysis. The recombinant baculovirus was transfected into sf9 cells by Cellfectin reagent, the expressed HA protein was analyzed by SDS-PAGE、Western-blot、HA test and HI test and it have good immunological activity.