中国生物工程杂志

Two ORFs in Staphylococcus aureus genomes were predicted to encode nucleases. One is encoded the nuclease A (SNase) without a gene name (termed nuc1); the other is encoded a thermonuclease (TNase) with a name of nuc (termed nuc2). To further investigate the function of nuc2 in S.aureus, the resulting plasmid, pBT2Δnuc1 was successfully constructed. The recombinant plasmid pBT2?nuc1 was introduced into S .aureus RN4220 by electroporation. Using the resistant marker for seven passages of culture, the recombination rate was about 2% (7/345). A nuc1-deleted mutant of S. aureus strain RN4220 (termed RN4220Δnuc1) was successfully constructed and conformed by PCR and RT-PCR.