中国生物工程杂志

Abstract:Enormous economic loses were caused by FMD which was a kind of virulent infections disease.This study was carried out to research the molecular immune mechanism of inactivated FMD vaccines, lay the foundations for invention of anti-FMDV medicine. In the research, mRNA differential display method was used to compare gene expression between PK-15 cells stimulated by Swine Foot and Mouth Disease and normal controls. 30 ESTs were obtained by extraction , amplification and purification. The 30 ESTs were identified by reverse northern dot bloting .8 positive DNA fragments were cloned and sequenced.All 8 ESTs were compared with nucleotide sequences deposited in the nr database and dbEST database of GenBank via BLASTn tool. E1 was found highly similar to Heat shock 60kDa　protein 1, E2 was found highly similar to Sus scrofa MHC class I SLA genomic region, haplotype H01,clone. E7 were extended and separated by searching database. The ORF database were used to analyse the extened bands. The result indicated that E7 was highly similar to arginase I of Sus scrofa. heat shock 60kDa protein 1, Sus scrofa MHC class I SLA genomic region haplotype H01 clone, arginase, type I of Sus scrofa and the other genes with unknown functions were regarded as candidate genes in anti-FMDV research, and their specific functions need to be explored in further study.