中国生物工程杂志

Abstract The proteomics technique platform of bovine spermatozoa was established by exploring the different sample preparation methods, the components of rehydration solution and optimizing the 2D electrophoresis protocols. The differential proteins between the fresh and frozen-thawed spermatozoa were also explored. The results indicated that the technique system with the high qualitative and stable 2D image had been obtained by preparing the bovine spermatozoa proteins with the heat TRIzol method and combining with the optimized 2D electrophoresis protocols. The differential in-gel electrophoresis showed that 20 protein spots were absent in quality, 2 spots decreased and 10 spots increased significantly in quantity after frozen-thawed. As a intermediate result, the 2D platform established in the research and the differential proteins found between fresh and frozen sperm will provide better basis for revealing the cryodamage mechanism of sperm cryopreservation and studying the differential proteomics of the X, Y spermatozoa.