中国生物工程杂志

ABSTRACT：Aim: To approach the relationship between cellular immune function and cerebrovascular disease(CVD) injury by estab1ish two-dimensiona1 electrophoresis(2-DE)maps of Splenolymphocyte in rat with intracerebral hemorrhage(ICH) and cerebral ischemia ,and identify the differentially expressed proteins．Methods: SD rats were randomly divided into normal group, intracerebral hemorrhage group (intracerebral hemorrhage induced by VII-collagenase and cerebral infarction group (a thread of a middle cerebral artery occlusion model). The Splenolymphocyte of rats were isolated and the total proteins were extracted and separated by 2-DE .The differentially expressed proteins were analyzed using PDQUEST analysis software，and then identified by peptide mass fingerprint(PMF)based on matrix-assisted laser desorption／ionization time of flight mass spectrometry (MALDI—TOF—MS). Resuits：Glia maturation factor gamma 、Rho, GDP dissociation inhibitor (GDI) beta 、Peroxiredoxin-II 、Hypothetical protein 、Beta-actin 、NADH dehydrogenase [ubiquinone] flavoprotein 2、60S acidic ribosomal protein P0、40S ribosomal protein SA were significantly increased, annexin III was significantly reduced in in rat with intracerebral hemorrhage and cerebral ischemia. Conclusions：The well-resolved and reproducible 2-DE pattern of Splenolymphocyte were established in rat with cerebral hemorrhage and cerebral ischemia and ten differentially expressed proteins were characterized.This approach may lay a foundation for the further investigation of the relationship between cellular immune function and cerebrovascular disease(CVD) injury.