中国生物工程杂志

Abstract Kosteletzkya virginica oil as raw materials was used to produce biodiesel. The single factor experiment and the orthogonal experiment combination design were adopted to study the effects of such factors as reaction temperature, catalyst dosage，mole ratio of methanol to Kosteletzkya virginica oil，reaction time and the rate of rotation on oil transesterification ratio. The results indicated the order of factors that influence the oil transesterification ratio within the experimental range was as follows: the rate of rotation r＞catalyst dosage＞mole ratio of methanol to Kosteletzkya virginica oil ＞reaction time＞reaction temperature. The optimal technological parameters of oil transesterification from Kosteletzkya virginica oil should be as follows: the rate of rotation 1800r.min-1, catalyst dosage 0.6%, mole ratio of methanol to Kosteletzkya virginica oil 6/1,reaction time 60min and reaction temperature 65℃, and under such conditions, the total oil transesterification ratio after transesterification three times is 97.8%.