Inhibition of K-RASAsn12 Expression by Vector-based RNA Interference in Human Pancreatic Cancer Cell Line

China Biotechnology

简报

Inhibition of K-RASAsn12 Expression by Vector-based RNA Interference in Human Pancreatic Cancer Cell Line

Download:

HTML

PDF(0KB) HTML
Export: BibTeX | EndNote (RIS)

Abstract To silence the expression of K-RASAsn12 in human pancreatic cancer cell line by vector-based RNAi(RNA interference) technique,two single-strand DNA sequences encoding mutant-specific shRNA (short haipin RNA) for K-RASAsn12 were synthesized and then inserted into pSilenCircle. The recombinant plasmid was called pSC-K-RASAsn12.According to the same method, pSC-GFP encoding shRNA for GFP was gained.Both recombinant plasmids were transfected into human pacreatic cancer cell line AsPC-1 and BxPC-3.The expression level of K-RASAsn12 was detected by semi-quantitative RT-PCR and Western Blot. The result indicated that the recombinant plasmid edcoding mutant-specific shRNA for K-RASAsn12 can inhibit significantly the expression of K-RASAsn12 without affection of wild-type K-RAS（K-RASWT）in Human Pancreatic Cancer Cell Line.

Key words： K-RAS Senescence-Accelerated Mouse P10/Ta shRNA (short haipin RNA) Pancreatic cancer

Received: 21 November 2005 Published: 25 April 2006

ZTFLH:	Ｑ782
	Ｑ786

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

. Inhibition of K-RASAsn12 Expression by Vector-based RNA Interference in Human Pancreatic Cancer Cell Line. China Biotechnology, 2006, 26(04): 86-90.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I04/86

No related articles found!

Viewed

Full text

Abstract

Cited

Shared

Discussed