Molecular Cloning and Characterization of mBolA1, a Novel Mouse Secreted Protein

China Biotechnology

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Molecular Cloning and Characterization of mBolA1, a Novel Mouse Secreted Protein

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Abstract AbstractUsing bioinformatical tools and experimental approaches, a novel secreted protein gene, mouse BolA1 was obtained . mBolA1 spans 730bp of cDNA and locates on mouse chromosome 3F2, which encodes a protein with 137 amino acids, mBolA1 has a conserved BolA domain and the PI is 9.05.The coding sequence of mBolA1 was cloned by RTPCR method from cDNA pool composed of mouse tissues cDNA. Western blot showed that mBolA1 was secreted out from the transiently transfected COS7 cells. Subcellular localization showed that mBolA1 protein located in cytoplasm and didn’t colocalized in Golgi, which indicate that mBolA1 is a unconventional secretion/nonclassical secretory protein. RTPCR result showed this gene was abundantly expressed in mouse tissues. The particular functions of this gene need further investigation.

Key words： mBolA1 nonclassical secretory Subcellular localization secreted protein

Received: 25 October 2005 Published: 25 February 2006

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Cite this article:

. Molecular Cloning and Characterization of mBolA1, a Novel Mouse Secreted Protein. China Biotechnology, 2006, 26(02): 0-0.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I02/0

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