Abstract In this paper, we have analyzed and discussed some aspects in the 2-dimensional gels electrophoresis such as the serum sample solubilization, the gel isoelectric focusing program ,the gel equilibration, the gel stain etc, generalized some problem in operation which must be stressed, and obtained the high resolution and good repetition results. It is referred to the serum sample analysis in 2-dimensional gels electrophoresis technology.
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