Protective effects of MaFGF on renal tubular epithelial cells damaged by cisplatin

China Biotechnology

2007, Vol. 27

Issue (6): 10-14 DOI:

Protective effects of MaFGF on renal tubular epithelial cells damaged by cisplatin

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Abstract

0bjective To investigate the protective effects of MaFGF on the injury of cultured renal tubular epithelial cells induced by DDP. Methods These primary cells were inoculated into 96 well plates. (1)After 72h culture，DDP with different concentration was added，and MaFGF with different concentration was added further as the experimental group after 12h of the DDP addition. WST-8 assays were performed to test the cell viability after 36h treated by MaFGF.（2）The injury model was induced by DDP. And the protective effect of MaFGF on the injured renal tubular epithelial cells was observed. Results (1)The concentration of DDP that inhibited 50% cell growth(IC50) was increased by MaFGF；(2)The biochemical and zymological results between the DDP group and the control group were different significantly. However，with the addition of MaFGF， the differences of SOD、GSH-Px enzyme activity between the two groups were insignificant，except for MDA and NO. Conclusion MaFGF could exert obvious protective effects on the renal tubular epithelial cells damaged by DDP

Key words： MaFGF cisplatin Renal tubular epithelial cell Cell culture

Received: 09 February 2007 Published: 25 June 2007

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. Protective effects of MaFGF on renal tubular epithelial cells damaged by cisplatin. China Biotechnology, 2007, 27(6): 10-14.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I6/10

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