中国生物工程杂志

Objective: To investigate the effect of high-concentration RA (all-trans retinoic acid，RA) on the differentiation of ESC. Methods: During the formation of embryoid bodys, exposured to 5μM RA. Neural precursor cells were replated in different substrates. Immunofluorescence analysed the different stage of ESC differentiation. Results: 5μM RA treatment of differentiating ESC was important for the induction of nestin positive cells. Approximately 76% of the cells were β-tubulin III positive, 55% of β-tubulin III positve cells were GABA positive when cultured on LPO, whereas only 4% were CHAT positive. Conclusion: High-concentration RA strongly drived neural induction.