| 技术与方法 |
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| Construction of cartilage using injectable fibrin sealant with human embryo cartilages cells in vivo. |
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Abstract Objective: To investigate the feasibility of Fibrin Sealant (FS) as a carrier of human embryo chondrocytes to create new cartilage in vivo. Methods: Chondrocytes isolated by collagenase digestion from the human embryo cartilage were cultured and expanded in monolayer system. The biomechanical properties of the chondrocytes were investigated. 1×107、2×107、3×107 fourth passage chondrocytes were mixed with FS respectively. Then the FS-chondrocyte mixture was injected into the dorsum of nude mice，the ability of chondrogenesis in vivo was investigated 10 weeks later. Results: The proliferation and secretion of substance of the third-fourth passage were powerful. There are specimens like cartilage were harvested in all experimental groups. The average wet weight and GAG content has increased with the increase of cell numbers planted. The difference between any two groups is significant (p<0.05, n=3). There is no significant difference in GAG content in 3×107 group and the normal embryo cartilage (p>0.05, n=3). When the specimens were sectioned and stained, it was demonstrated that they hooked like normal cartilage tissue, with chondrocytes lying in a lacunae in basophilic ground-glass substance around it. The Alcian blue staining and Type II Collagen expressions were strong positive. Most cells appeared to contain several golgi zones and large areas of rough endoplasmic reticulum. Conclusion: This study demonstrates that FS and human embryo cartilage cell can be used as a constructing technique of tissue engineered injectable cartilage.
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Received: 24 May 2005
Published: 25 August 2006
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