中国生物工程杂志

Objective: To investigate effects of bFGF and collagen on tissue-engineered cartilage in pellet culture. Methods: Growth-plate chondrocytes were incubated in pellet culture. bFGF and Collagen was applied alone or together based on the results of previous research.The histological structure of neocartilage was observed by stained with H＆E and Immunohistochemical analysis. The DNA quantity of neocartilage was measured by hoechst33258 dye.The production of matrix was estimated based on hydroxyproline quantity and Alcian Blue stain method. Results: The histological structure of in vitro-formed tissue was similar to growth-plate cartilage.The DNA quantity of neocartilage increased by the supplement of bFGF and collagen. The production of matrix was promoted by collagen. bFGF and collagen suppressed the expression of collagen Ⅰ,and decelerated the decrease of collagenⅡ. The combined usage of bFGF and collagen had synergistic effects. Conclusion: Pellet culture maintained the phenotype of chondrocyte and promoted matrix production. bFGF and Collagen improved the construction of engineered cartilage. The combined usage of bFGF and collagen had synergistic effects on the regeneration of cartilage.