Sequence Analysis of Coat Protein Gene of Cucumber Mosaic Virus Isolate Infecting Basella rubra L.

China Biotechnology

2008, Vol. 28

Issue (2): 96-100 DOI:

Sequence Analysis of Coat Protein Gene of Cucumber Mosaic Virus Isolate Infecting Basella rubra L.

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Abstract

Cucumber mosaic virus was detected from infected Basella rubra L. with the indirect enzyme-linked immunosorbent assay. Total RNA was extracted from infected leaves and the cDNAs of coat protein gene of CMV-Ba were obtained by RT-PCR. The amplified cDNA fragments were then cloned into pMD 18-T vector and sequenced，the result showed that the CP gene was 657 nucleotides in length. This sequence was aligned with the obtained CP gene and some CMV strains or isolates of subgroup Ⅰ and subgroup Ⅱ in GenBank using DNA MAN software. The results showed that CMV-Ba shared 90.9% -93.8% and 76.1%-76.9% identity with the known CP genes of subgroup Ⅰ and Ⅱ respectively in nucleotide level，on the other hand，amino acids deduced from CMV-Ba CP gene shared 92.7% -97.7% and 72.4%-78.1% identity with the known CP protein of subgroup Ⅰ and Ⅱ，respectively. This suggested that CMV-Ba CP gene belongs to CMV subgroupⅠ.

Key words： Basella rubra L. Cucumber mosaic virus coat protein gene Sequence analysis

Received: 12 October 2007 Published: 25 February 2008

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. Sequence Analysis of Coat Protein Gene of Cucumber Mosaic Virus Isolate Infecting Basella rubra L.. China Biotechnology, 2008, 28(2): 96-100.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I2/96

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