Immunogenicity in a prime–boost regimen of a modified vaccinia virus Ankara (MVA) vectored experimental HIV-1 clade C/B’ vaccine

China Biotechnology

2008, Vol. 28

Issue (2): 10-15 DOI:

Immunogenicity in a prime–boost regimen of a modified vaccinia virus Ankara (MVA) vectored experimental HIV-1 clade C/B’ vaccine

Gao Yan

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Abstract

Abstract Objective To evaluate the immunogenicity of HIV-1 clade C/B’ vaccine based on modified vaccinia virus Ankara (MVA) vector in mice. Methods Mice were inoculated with 3-dose HIV vaccine by intramuscular injection. Blood sample were collected every second week, and then the antibodies against HIV were detected. At week 6, mice were killed and cellular immune responses were examined by ELISPOT. Result The number of spot forming cells in the 107 pfu/mL -dose group was more than those of 105 pfu/mL -dose and 106 pfu/mL -dose groups significantly. HIV specific antibodies emerged at week 2 and elevated rapidly at week 4 and week 6. The level of specific IgG in the 107 pfu/mL -dose group was more than those of 105 pfu/mL -dose and 106 pfu/mL -dose groups significantly. Conclusion The ADMVA induces both humoral immunoresponse and cellular immune responses.

Key words： HIV-1 clade C/B MVA Vaccine Immunogenicity

Received: 12 November 2007 Published: 25 February 2008

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Cite this article:

Gao Yan . Immunogenicity in a prime–boost regimen of a modified vaccinia virus Ankara (MVA) vectored experimental HIV-1 clade C/B’ vaccine. China Biotechnology, 2008, 28(2): 10-15.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I2/10

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