中国生物工程杂志

The matrix protein (M) of human respiratory syncytial virus (RSV) is important for virion morphogenesis and contains CTL epitopes valuable to the research on RSV vaccine development. Therefore, M gene was amplified from RSV infected HEp-2 cells by RT-PCR, and FGAd/RSVM of the replication deficient recombinant adenovirus containing M gene was constructed and identified. DNA sequencing displayed no nonsense mutation in M gene. From the FGAd/RSVM infected 293 cells, the transcription and expression of M gene were confirmed by RT-PCR, indirect immunofluorescence assay and Western blotting, respectively. The resulting FGAd/RSVM laid a solid foundation for the investigation into the protective immunity against RSV infection and its mechanism.