中国生物工程杂志

We used Uniform Design to optimize some relative factors influencing the expressing of t-PA　variant-rPA(K) in E.coli system, and found the best expressive conditions. They were(by using 300 mL culture fask): the volume of media was 25 mL, inducing time was 5.3 hours, pH was 6.0, the concentration of IPTG was 0.1 mmol/L, inducing time was 25?C, and best culture media was HD. After being optimized, the yield of expression had been improved from 0.16 to 0.48, and it was as 3 times as before. The results above will offer the basement for purification and renaturation of rPA(K).