Studies on Two-stage pH Control Strategy of Recombinant Cutinase Production

China Biotechnology

2008, Vol. 28

Issue (5): 59-64 DOI:

Studies on Two-stage pH Control Strategy of Recombinant Cutinase Production

Jian Chen

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Abstract

To enhance the yield and productivity of cutinase from recombinant Bacillus subtilis WSHB06-07, the effect of pH on cell growth and product formation was investigated. Based on specific cell growth rate and specific cutinase formation rate under different pH condition, a two stage pH control strategy was developed, in which pH was controlled at 7.5 for the first 4h and then shift to 6.5. By the utilization of this strategy, the yield of cutinase was significantly improved, the maximal cutinase activity and the productivity were 170 U/mL and 16.9 kU/(L·h), which were increased by 122.6% and 123.2%, respectively, compared to that from the condition of constant pH 7.5.

Key words： recombinant cutinase Bacillus subtilis pH two-shift strategy specific cell growth rate specific cutinase formation rate

Received: 31 January 2008 Published: 25 May 2008

ZTFLH:

TQ925.6

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Cite this article:

Jian Chen . Studies on Two-stage pH Control Strategy of Recombinant Cutinase Production. China Biotechnology, 2008, 28(5): 59-64.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I5/59

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