中国生物工程杂志

This paper reported the role of Phosphoenolpyruvate Carboxylase of procaryote to the genetic regulation of lipids synthesis. A gene fragment (pepcA) containing the conservative sequence (Fa) of PEPC gene from filamentous cyanobacterium Anabaena sp. PCC 7120 was cloned and inserted between BamHI sites of the shuttle expression vector pRL-489 in forward or reversely orientation. The recombinants (pRL-Sen pepcA and pRL-Anti pepcA) were transferred into the host cells Escherichia coli DH5a. The results showed that the introduction of pepcA fragment hadn't obvious influence to the growth of transgenic E.coli. Compared to the wide cells, the enzyme activity of the transgenic E.coli of pRL-Anti pepcA reduced to 30.2%, the protein content of that was 23.6% lower than in wide cells, and the lipid content of that increased 46.9%. While the enzyme activity of the transgenic E.coli of pRL-Sen pepcA was 2.38 fold of wide cells, the protein content of that increased 14.5%，and the lipid content was lower 49.6% than in wide type cells; The content of C18 in transgenic E.coli increased obviously. The above results may offer a clue to produce biodiesel with procaryote.