Clone, Expression and Immunological Analysis of Neisseria meningitidis Outer Membrane Protein NhhA

China Biotechnology

2010, Vol. 30

Issue (04): 8-12 DOI:

Clone, Expression and Immunological Analysis of Neisseria meningitidis Outer Membrane Protein NhhA

CHEN Liu,LI Jian-feng,XU Wei-ming,DAI Zong-xiang,LI Zhi-hua,SUN Qiang-ming

Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China

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Abstract

Neisseria meningitidis gene GNA0992 was cloned into the prokaryotic expression vector pET-20b, and soluble expressed in E.coli BL21 (DE3). Purified NhhA stimulated a significant antigen-specific humoral response following three intraperitoneal immunization, while bactericidal assay showed that NhhA can elicit antibodies capable of inducing bactericidal activity against group B N.meningitidis strains. The results indicate that NhhA is a good candidate for vaccine development.

Key words： Neisseria meningitides NhhA Prokaryotic expression Immunological property

Received: 18 November 2009 Published: 29 April 2010

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	CHEN Liu
	LI Jian-Feng
	XU Wei-Meng
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	LI Zhi-Hua
	SUN Jiang-Meng

Cite this article:

CHEN Liu, LI Jian-Feng, XU Wei-Meng, DAI Zong-Xiang, LI Zhi-Hua, SUN Jiang-Meng. Clone, Expression and Immunological Analysis of Neisseria meningitidis Outer Membrane Protein NhhA. China Biotechnology, 2010, 30(04): 8-12.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2010/V30/I04/8

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