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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (7): 79-84    DOI:
    
Construction of the Monascus aurantiacus As3.4384orf7Gene Deletion Strains and Functional Analysis
ZOU Le-hua, LI Yan-ping, HUANG Zhi-bing, XU Yang
State Key Laboratory of Food Science and Technology, Sino-Germany Joint Research Institute,Nanchang University,Nanchang 330047,China
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Abstract  

Monascus can produce several kinds of beneficial secondary metabolites. But Monascus can also produce citrinin that is harmful to mammals because of nephrotoxic and hepatoxic activity. Thus it is essential to control the production of this toxin in order to increase the safety of Monascus-related product. Extensive research on citrinin pathway and genes involving in this toxin biosynthesis is conducted. Six genes responsible for citrinin biosynthesis are clusted within 21kb DNA region. A gene(orf7) which locates in the vicinity of orf5 was cloned. The orf7 gene disruption mutants in M. aurantiacus As3.4384 were constructed. The citrinin yield of the orf7 gene disruption mutants was analyzed by HPLC and the pigments were analyzed by UV spectrophotometer. From 13th to 19th day of cultivation, the yield of citrinin of orf7 disruptant increased 142.4% compared to wild-type As3.4384. So it was confirmed that orf7 was related to metabolism of citrinin.



Key wordsMonascus aurantiacus As3.4384      orf7 gene      Gene-knockout      Citrinin     
Received: 24 March 2011      Published: 25 July 2011
ZTFLH:  Q78  
Cite this article:

ZOU Le-hua, LI Yan-ping, HUANG Zhi-bing, XU Yang. Construction of the Monascus aurantiacus As3.4384orf7Gene Deletion Strains and Functional Analysis. China Biotechnology, 2011, 31(7): 79-84.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I7/79


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