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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (7): 60-64    DOI:
    
Screening of Camptothecin Production and SRAP Analysis of Endophytic Fungi from Camptotheca acuminata Decne
LI Xia1, LIU Jia-jia1, CHEN Jian-hua2, LUAN Ming-bao2, YIN Zhen-zhen1, YANG Dong-liang1
1. School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China;
2. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410083, China
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Abstract  

Endophytic fungi, lived in stems and leaves of host plants, were supposed to be effective or novel sources for therapeutic compounds. Camptotheca acuminata Decne is a traditional medical plant in China. About 50 endophytic fungi were isolated from barks of C. acuminata. A new fungus producing camptothecin (CPT) was identified as a Penicillium based on its morphological features. Ten endophytic fungi of C. acuminata were chosen to be amplified using molecular maker sequence related amplified polymorphism (SRAP). The combinations of SRAP primers turned out to be polymorphic and a total of 1 295 polymorphic bands were obtained. These strains were grouped into 3 main clusters and revealed that SRAP could isolate the strains of C. acuminata efficiently. It also provided evidence for discussing the genetic diversities of species in C. acuminata with SRAP molecular method.



Key wordsEndophytic fungus      Penicillium      Molecular maker      Genetic diversity     
Received: 02 April 2011      Published: 25 July 2011
ZTFLH:  Q75  
Fund:  

Supported by the National Natural Science Foundation of China (30900913) and the Key Research Items from the Ministry of Education of China (03126)

Cite this article:

LI Xia, LIU Jia-jia, CHEN Jian-hua, LUAN Ming-bao, YIN Zhen-zhen, YANG Dong-liang. Screening of Camptothecin Production and SRAP Analysis of Endophytic Fungi from Camptotheca acuminata Decne. China Biotechnology, 2011, 31(7): 60-64.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I7/60


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