D-tagatose Production Utilizing Immobilized Recombinant <em>Escherichia coli</em> cells

China Biotechnology

2011, Vol. 31

Issue (7): 85-90 DOI:

D-tagatose Production Utilizing Immobilized Recombinant Escherichia coli cells

FU Feng-gen, XU Zheng, LI Gui-xiang, LI Sha, FENG Xiao-hai, XU Hong

State Key Laboratory of Materials-Oriented Chemical Engineering,College of Food Science and Light Industry, Ningjing University of Technology,Nanjing 210009,China

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Abstract

Recombinant E.coli cells were immobilized with calcium alginate for the isomerization of D-galactose to D-tagatose. The influences of immobilization process and reaction conditions on the catalytic efficiency and D-tagatose production stability of the immobilized cells are investigated. The optimal conditions were as follows: the temperature, pH and mental ion were 65℃,6.5 and 1mmol/L Mn²⁺, respectively. The optimal substrate (D-galactose) and cell concentrations of 100 g/L and 40 g/L were selected, respectively. Stability of the alginate beads under high temperatures was enhanced after cross-linked by 0.3% glutaraldehyde for 30min. The effect of molar ratio between borate and substrate on isomerization was studied, it was found that borate could change the initial chemical equilibrium of D-galactose isomerization and lead to a high production of D-tagatose. After conversion for 24h, the highest conversion rate for D-tagatose using D-galactose as the substrate reached to 65.8% by immobilized recombinant E.coli cells, and the average conversion rate of eight repeated batch conversions was 60.6%, which laid the foundation for industrial production of D-tagatose.

Key words： D-tagatose Immobilized cells Recombinant E.coli Borate L-arabinose isomerase

Received: 17 March 2011 Published: 25 July 2011

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	FU Feng-gen
	XU Zheng
	LI Gui-xiang
	LI Sha
	FENG Xiao-hai
	XU Hong

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FU Feng-gen, XU Zheng, LI Gui-xiang, LI Sha, FENG Xiao-hai, XU Hong. D-tagatose Production Utilizing Immobilized Recombinant Escherichia coli cells. China Biotechnology, 2011, 31(7): 85-90.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2011/V31/I7/85

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