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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (06): 29-37    DOI:
    
D-Type Cyclin Family in Populus: Functional Characterization and Expression Profiling under Phytohormones and Sucrose Treatment
HAO Shuang, XIA Xin-li, YIN Wei-lun
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
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Abstract  

The G1/S transition is one of the key regulatory points during the plant cell cycle. D-type cyclins (CYCDs) play critical roles in controlling the progression through the G1 into the S phase. Since CYCDs are responsive to stimulatory signals, they are functional in integrating mitogenic signals into cell division. However, the expression patterns and functional characteristics of different CYCD families in woody species remain to be elucidated. Six putative CYCD genes designated as PdCYCD1-7 from a hybrid poplar clone (P.deltoides × P.nigra, NE19) by complementing a yeast mutant lacking G1 cyclins were identified. But their effects on promoting yeast cell division after ectopic expression are divergent. Sucrose and phytohormone treatments on in vitro-grown poplar seedlings led to the alteration of morphological traits in roots. The effects on the transcript levels of PdCYCDs were monitored. Differential expression characteristics of the CYCDs in response to the mitogen supply were observed. Though CYCDs were induced by sucrose, their extents of induction among CYCD subgroups varied. PdCYCD6;4 and PdCYCD3;1 may have wider roles in responding to external signals than others, for they were modulated with sucrose and most hormones treatments. The induction of PdCYCD2;1 and PdCYCD1;1 by hormones depended on the presence of sucrose. PdCYCD5;1 was greatly stimulated by ethylene and the strengthening effect on induction was observed when sugar and hormones were added together. PdCYCD7;1 was not so sensitive to sucrose but was upregulated by gibberellin and ethylene. Together, these results suggest that the six populus CYCD genes identified here are functional in rescuing yeast cyclin mutant but may still have group-specific functions during the development of plants.



Key wordsCYCD Functional characterization      Sugar      Phytohormone      Poplar     
Received: 03 March 2011      Published: 28 June 2011
ZTFLH:  Q819  
Fund:  

This work was supported by the National Basic Research Program of China ("973" Programme, grant number 2009CB119101), National Natural Science Foundation of China (30730077, 30972339, 31070597), Program for New Century Excellent Talents in University of China (NCET-07-0083), the Hi-Tech Research and Development Program of China (2007AA10Z106).

Corresponding Authors: Shuang Hao, YIN Wei-lun     E-mail: shuangshuangxixi@gmail.com; yinwl@bjfu.edu.cn
Cite this article:

HAO Shuang, XIA Xin-li, YIN Wei-lun. D-Type Cyclin Family in Populus: Functional Characterization and Expression Profiling under Phytohormones and Sucrose Treatment. China Biotechnology, 2011, 31(06): 29-37.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I06/29

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