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| Adult Stem Cells Harvested by Implanting Gelatin Sponges into Spatium Intermusculare |
| WU Xiao-yun1, WANG Shi-li1, MU Zhong-xiang2 |
1. Key Laboratory for Biotech-Drugs Ministry of Health Shandong Medicinal Biotechnology Center, Jinan 250062, China;
2. Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China |
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Abstract A new method to harvest stem cells was established by implanting biomaterials into mice. Gelatin Sponges (GS) were implanted into the spatium intermusculare of mice hind limbs. A large number of migrating cells were isolated from the transplanted biomaterials at 12 days after implanting. The characteristics of adherent cells were similar to that of bone marrow (BM) stem cells, including morphology, proliferation potential and multilineage differentiation capacity. The frequency of CFU-F in GS (82.2±10.6/105) was much higher than in BM (43.7±7.4/106) (P<0.05). In additional, BM transplantation demonstrated that stem cells in the GS originated from the PB. Moreover, it is possible for autologous cell therapy, so this method may be a promising alternative for the clinical application.
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Received: 23 November 2010
Published: 28 June 2011
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| [1] Sherwood R I, Christensen J L, Conboy I M, et al. Isolation of adult mouse myogenic progenitors: functional heterogeneity of cells within and engrafting skeletal muscle. Cell, 2004, 119(4):543-554.
[2] Phillips M I, Tang Y L. Genetic modification of stem cells for transplantation. Adv Drug Deliv Rev, 2008, 60(2):160-172.
[3] Trounson A. New perspectives in human stem cell therapeutic research. BMC Med, 2009, 7:29.
[4] Niemeyer P, Fechner K, Milz S, et al. Comparison of mesenchymal stem cells from bone marrow and adipose tissue for bone regeneration in a critical size defect of the sheep tibia and the influence of platelet-rich plasma. Biomaterials, 2010, 31(13):3572-3579.
[5] de Villiers J A, Houreld N, Abrahamse H. Adipose derived stem cells and smooth muscle cells: implications for regenerative medicine. Stem Cell Rev Rep, 2009, 5(3):256-265.
[6] Wu X, Wang S, Chen B, et al. Muscle-derived stem cells: isolation, characterization, differentiation, and application in cell and gene therapy. Cell Tissue Res, 2010, 340(3):549-567.
[7] Caplan A I. The mesengenic process. Clin Plast Surg, 1994, 21(3):429-435.
[8] Huang J I, Kazmi N, Durbhakula M M, et al. Chondrogenic potential of progenitor cells derived from human bone marrow and adipose tissue: a patient-matched comparison. J Orthop Res, 2005, 23(6):1383-1389.
[9] 安新玲,韩金祥,王世立. 重组人骨形态发生蛋白-7活性检测方法的建立. 中国生物制品学杂志, 2010, 23(12):1357-1360. An X L, Han J X, Wang SL. Chinese J of Biological, 2010, 23(12):1357-1360.
[10] Li J, Wang S, Han J, et al. Cells captured from spatium intermusculare by porous material exhibit the characteristics of stem cells. Histochem Cell Biol, 2008, 130(4):741-748.
[11] Badylak S F, Freytes D O, Gilbert T W. Extracellular matrix as a biological scaffold material: Structure and function. Acta Biomater, 2009, 5(1):1-13.
[12] 丁宁,王世立,韩金祥,等. 组织工程材料捕获成体干细胞的影响因素研究. 中国医药生物技术, 2009, 4(3):171-176. Ding N, Wang S L, Han J X, et al. Chinese Medicinal Bintechnology, 2009, 4(3):171-176.
[13] 李海生, 陈金武, 朱玲玲,等. 持续低氧增强人骨髓间充质干细胞体外增殖. 基础医学与临床, 2005, 25(3):268-271. Li H S, Chen J W, Zhu L L. Basic & Clinical Medicine, 2005, 25(3):268-271.
[14] Soleimani M, Nadri S. A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. Nat Protoc, 2009, 4(1):102-106.
[15] 王心蕊,何旭,王医术,等. 低氧促进人骨髓间充质干细胞迁移的实验研究. 中国免疫学杂志, 2006, 22(12):1100-1102. Wang X R, He X, Wang Y S, et al. Chinese J of Immunology, 2006, 22(12):1100-1102.
[16] Lennon D P, Edmison J M, Caplan A I. Cultivation of rat marrow-derived mesenchymal stem cells in reduced oxygen tension: effects on in vitro and in vivo osteochondrogenesis. J Cell Physiol, 2001, 187(3):345-355.
[17] Peault B, Rudnicki M, Torrente Y, et al. Stem and progenitor cells in skeletal muscle development, maintenance, and therapy. Mol Ther, 2007, 15(5):867-877.
[18] Issarachai S, Priestley G V, Nakamoto B, et al. Cells with hemopoietic potential residing in muscle are itinerant bone marrow-derived cells. Exp Hematol, 2002, 30(4):366-373.
[19] Chapel A, Bertho J M, Bensidhoum M, et al. Mesenchymal stem cells home to injured tissues when co-infused with hematopoietic cells to treat a radiation-induced multi-organ failure syndrome. J Gene Med, 2003, 5(12):1028-1038. |
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