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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (06): 106-110    DOI:
    
Preparation and Identification of the Specific Antibody against Diethylstilbestrol
DENG Sheng-liang1,2, LI Ping1, LIU Hong-bin1, CHENG Ma-li3, YU Hong-xia1, CHEN Gang1, YANG Shu-ming1
1. Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agriculture Sciences, Beijing 100081, China;
2. Institute of Microbiology, Jiangxi Academy of Sciences, Nanchang 330029, China;
3. Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China
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Abstract  

Diethylstilbestrol was activated by ethyl 4-bromobutyrate to introduce carboxyl group and then conjugated to BSA via EDC/NHS as immunogen, New Zealand rabbits were immunized and antisera were prepared. The results showed that complete antigen was successfully synthesized and specific antibody was generated, with titer of 1.28×105 the antisera cross-reactivity with hexestrol (9.34%), dienestrol (1.1%) and no cross-reactivity with 17β-estradiol. An indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed for determination of DES. In the linear range between 1 and 30 ng/ml, IC50 of 7 ng/ml and a detection limit of 0.3 ng/ml for DES were reached. Therefore the assay as screening method can meet the demand of DES residue detection for animal food products.



Key wordsDiethylstilbestrol      Specific antibody      ELISA     
Received: 30 November 2010      Published: 28 June 2011
ZTFLH:  Q819  
Cite this article:

DENG Sheng-liang, LI Ping, LIU Hong-bin, CHENG Ma-li, YU Hong-xia, CHEN Gang, YANG Shu-ming. Preparation and Identification of the Specific Antibody against Diethylstilbestrol. China Biotechnology, 2011, 31(06): 106-110.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I06/106

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