中国生物工程杂志

On the base of element and metabolism balancing, the mathematic model of the human-like collagen expression phase with recombinant Escherichia coli BL21 was developed and the unknown parameters in the model were estimated with the method of nonlinear optimization. The model was in agreement with the growth kinetics and the metabolic kinetics, and the key calculated parameters of αh, αp and mx were 1.173 molo C-mol-1, 293.814 molo C-mol-1 and 17.878 molo C-mol-1oh-1 respectively. This model could preferably predict the macroscopic reaction rates, and in the synthesis phase of human-like collagen, the specific growth rate should be controlled at 0.04 h-1 with controlling glucose feeding rate to gain the highest specific production rate of human-like collagen.