Abstract High abundant plasma proteins deterior the effect of seperating plasma proteins in plasma proteomics research, it is necessary to seperate high abundant proteins in plasma samples. Protein G were used to absorbe the anti-albumn monoclonal antibodies then cross-linked with mAb by Dimethyl pimelinediimidate chloride. The resulst suggested that 1000µg antibody were site-directed immoblized and kept the capacity of binding around 600µg albumin proteins after 10 cycles of elution-regeneration-binding.. The bound proteins were identified with 2-DE-MALDI-TOF/TOF. Bound proteins included albumin, IgG, Vitamin D binding protien, Fibrinogen, Transthyretin, Keratin 10, Transferrin, C3, Proapolipoprotein, CD5 antigen-like, Alpha1-Antitrypsin. Our study showed a prevailing,applicable and valuable approach to separate high abundant proteins in human plasma as well as other samples.
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