中国生物工程杂志

Gentamicin was a broad-spectrum aminoglycoside antibiotic. It not noly was used on the clinic to cure, but also applied to the stockbreeding widely. By reason of fermentation cycle long, production rate low and production costs high, so amelioration production method was imperative under the situation. The paper reported that added a little quantity of lysine, glycine, tyrosine, methionine into the medium of gentamicin producing Micromonospora purpurea during inoculation could improve the metabolic cabability of microbe, shorten fermentation cycle and increase production rate.of gentamicin. The new method was successfully developed in shaking flask test and applied to 5L glass fermentor for one month. Average data of one month showed that the fermentation time per cycle was shortened about 40% and the yield of gentamicin per fermentor increased about 14% in comparison with the conventional technique. The production rate of gentamicin was increased 30% ~950% (according to the capability of strain). The quality of the end product ——gentamicin conformed to CP2000, BP2000 and USP26 pharmacopoeia. Therefore, the new method remarkably may reduce production costs of gentamicin, show strong competitiveness in the marketplace.