Study of expression of Pneumococcal surface protein and Immunogenicity

China Biotechnology

2008, Vol. 28

Issue (4): 65-69 DOI:

Study of expression of Pneumococcal surface protein and Immunogenicity

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Abstract

The specific fragment of Pneumococcal surface protein A( PspA) and Pneumococcal Surface Adhesin A(PsaA) gene was amplified by PCR from Streptococcus pneumonia 5. The amplified fragnent of PspA and PsaA gene was ligated into pET- 27b (+) vector and transformed into BL 21 E.coli for expression and obtain the expressive production of PspA and PsaA. Induced by IPTG, the expression level was as high as 75 % of total protein. The result showed that the recombinant plasmid could express a specific 75 kDa and 37 kDa fusion protein in E. coli BL 21, which showed the good immunogenicity and a broadly cross reactivity with the other serotypes.

Key words： PspA gene PsaA gene immunogenicity prokaryotic expression

Received: 02 November 2007 Published: 25 April 2008

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R5l7.201

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. Study of expression of Pneumococcal surface protein and Immunogenicity. China Biotechnology, 2008, 28(4): 65-69.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I4/65

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