中国生物工程杂志

An eukaryotic expression plasmids pEGFP-C1-A3I3,with open reading frame(ORF) of FMDV WFL strain, was constructed based on the pEGFP-C1.The results of transient expression showed that the eukaryotic expression plasmids can be expressed in BHK-21.When cotransfected the plasmid with RNA transcribed from linearized cDNA clone,FMDV antigen and nucleotide acids was detected in cell culture fluid by sandwich ELISA and RT-PCR,respectively.And the virus particles were also found by electron microscope.The results proved that cotransfection of eukaryotic expression plasmid with RNA can improve virus rescuing.And for the first time we successfully rescued FMDV by cotransfecting eukaryotic expression plasmid with RNA transcribed in vitro.