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Gene expression analysis of a late embryogenesis abundant gene from Salvia miltiorrhiza Bunge |
LIU Cong-ling,WANG Zhe-zhi |
Colledge of Life Scences, Shaanxi Normal University, Key Laboratory of Medicinal Plant Resources and Natural Pharmaceutical Chemistry,Ministry of Education,National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, Xi’an 710062,China |
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Abstract According to the sequence of SmLEA gene, a DNA fragment of 1 038 bp upstream of the coding sequence of SmLEA gene was amplified by DNA walking with the genomic DNA of Salvia miltiorrhiz as the template. Sequence analysis showed that the fragment contained some putative ciselements relating to abiotic stress, ABA, seed specific expression.So the S.miltiorrhiz seedings was treated with 100μmol/L ABA,200mmol/L NaCl,4℃, and subjected to dehydration. The real-time PCR showed that expression levels of SmLEA was increased obviously, which was in accordance with the sequence analysis..
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Received: 22 September 2009
Published: 27 January 2010
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